A prerequisite to successfully conducting meaningful RNAi experiments are, on the one hand efficient and validated gene knockdown
, on the other hand the use of appropriate controls to exclude off-target effects.
SIRION Biotech offers the custom generation of individual RNAi control cell models to confirm ON-target phenotypes.
cell models enable the depletion of an endogenous target X while simultaneously overexpressing the ectopic, undegradable form of target X, functionally rescuing the specific phenotype.
The validity of VariCHECK(TM)
as RNAi ON-target control has been successfully demonstrated for the functional analysis of the oncology target GOIx.